Comparative Study of Antibacterial and Antifungal Activities of Silver Nanoparticles and Capsicum Annum Leaves Extracts
Keywords:
Capsicum annum leaves, Silver Nanoparticles, Antibacterial, AntifungalAbstract
Researchers are becoming more interested in the eco-friendly bio-synthesis of Ag-NPs because they could be used in many areas of science and technology. The Capsicum annuum L. extract was used to prepare silver nanoparticles (Ag-NPs) by treating silver nitrate with it. To make the Ag-NPs, a water-based extract of capsicum annum leaves was used to change the Ag+ ions in silver nitrate to Ag0.The synthesized particles was characterized using SEM, EDX, FTIR, and UV visible spectroscopy. The synthesized particles and extract were then tested for their ability to kill bacteria and fungi by testing them against species like Escherichia coli, Pseudomonas florescens, Bacillus subtilis, Staphylococcus aureus, Pseduomonousaliginoza, Candida albicans, Rhizopus oryzae, Aspergillus Niger, Aspergillus Parasiticus, and Aspergillus flavus. The comparison of antibacterial and antifungal activity against microbes was carried out and it was found from the growth curves of bacteria and fungi that the silver nanoparticles showed more zone of inhibition than the plant extract.
References
. Jiang ZJ, Liu CY, and Sun LW. (2005). Catalytic properties of silver nanoparticles supported on silica spheres. J. Phys. Chem. B. 109(5): 1730-1735.
. Okafor F, Janen A, Kukhtareva T, Edwards V, and Curley M. (2013). Green synthesis of silver nanoparticles, their characterization, application and antibacterial activity. Int. J. Environ. Res. Public Health. 10(10): 221-5238.
. Faraz A, Faizan M, Sami F, Siddiqui H, Pichtel J, and Hayat S. (2019). Nanoparticles: biosynthesis, translocation and role in plant metabolism. IET Nanobiotechnol. 13(4): 345-352.
. Chaloupka K, Malam Y, and Seifalian, AM, (2010). Nanosilver as a new generation of nanoproduct in biomedical applications. Trends Biotechnol. 28(11): 580-588.
. Sathishkumar G, Gobinath C, Karpagam K, Hemamalini V, Premkumar K, and Sivaramakrishnan S. (2012). Phyto-synthesis of silver nanoscale particles using Morinda citrifolia L. and its inhibitory activity against human pathogens. Colloids Surf. B. 95: 235-240.
. Qidwai A, Kumar R, and Dikshit A. (2018). Green synthesis of silver nanoparticles by seed of Phoenix sylvestris L. and their role in the management of cosmetics embarrassment. Green Chem. Lett. Rev. 11(2): 176-188.
. Ozen T, and Demirtas I. (2015). Antioxidative properties of Thymus pseudopulegioides: comparison of different extracts and essential oils. J. Essent. Oil-Bear. Plants. 18(2): 496-506.
. Yin IX, Zhang J, Zhao IS, Mei ML, Li Q, and Chu CH. (2020). The antibacterial mechanism of silver nanoparticles and its application in dentistry. Int J Nanomedicine. 555-2562.
. Karan T, Erenler R, and Bozer BM. (2022). Synthesis and characterization of silver nanoparticles using curcumin: cytotoxic, apoptotic, and necrotic effects on various cell lines. Z. Naturforsch. C. 77(7-8): 343-350.
. Türkmen N, Öz A, Sönmez A, Erol T, Gülümser D, Yurdakul B, Kayır Ö, Elmastas M, and Erenler R. (2014). Chemical composition of essential oil from Rosmarinus officinalis L. leaves. J. New Results Sci. 3(6): 27-31.
. Elmastas M, Erenler R, Isnac B, Aksit H, Sen O, Genc N, and Demirtas I. (2016). Isolation and identification of a new neo-clerodane diterpenoid from Teucrium chamaedrys L. Nat. Prod. Res. 30(3): 299-304.
. Jadhav K, Deore S, Dhamecha D, Hr R, Jagwani S, Jalalpure S, and Bohara R. (2018). Phytosynthesis of silver nanoparticles: characterization, biocompatibility studies, and anticancer activity. ACS Biomater. Sci. Eng. 4(3): 892-899.
. GEÇER EN, and ERENLER R. (2022). Biosynthesis of silver nanoparticles using Dittrichia graveolens (Asteraceae) leaves extract: characterisation and assessment of their antioxidant activity. Turk J Biod. 5(1): 50-56.
. ERENLER R, GEÇER EN, Nusret GENÇ, and YANAR D. (2021). Antioxidant activity of silver nanoparticles synthesized from Tagetes erecta L. leaves. International J. Chem. Technol. 5(2): 141-146.
. Gecer EN, Erenler R, Temiz C, Genc N, and Yildiz I. (2022). Green synthesis of silver nanoparticles from Echinacea purpurea (L.) Moench with antioxidant profile. Part. Sci. Technol.40(1): 50-57.
. Erenler R, and Dag B. (2022). Biosynthesis of silver nanoparticles using Origanum majorana L. and evaluation of their antioxidant activity. Inorg. Nano-Met. Chem. 52(4): 485-492.
. Erenler R, and Gecer EN. (2022). Synthesis of silver nanoparticles using Sideritis montana L. leaf extract: Characterization, catalytic degradation of methylene blue and antioxidant activity. Nano Res. 75: 17-28.
. Sahin Yaglioglu A, Erenler R, Gecer EN, and Genc N. (2022). Biosynthesis of silver nanoparticles using Astragalus flavesces leaf: identification, antioxidant activity, and catalytic degradation of methylene blue. J. Inorg. Organomet. Polym. Mater. 32(10): 3700-3707.
. Młynarczyk K, Walkowiak-Tomczak D, and Łysiak GP. (2018). Bioactive properties of Sambucus nigra L. as a functional ingredient for food and pharmaceutical industry. J. Funct. Foods. 40: 377-390.
. Gardea-Torresdey JL, Gomez E, Peralta-Videa JR, Parsons JG, Troiani H, and Jose-Yacaman M. (2003). Alfalfa sprouts: a natural source for the synthesis of silver nanoparticles. Langmuir. 19(4): 1357-1361.
. Rautaray D, Sanyal A, Bharde A, Ahmad A, and Sastry M. (2005). Biological synthesis of stable vaterite crystals by the reaction of calcium ions with germinating chickpea seeds. Cryst. Growth Des. 5(2): 399-402.
. Shankar SS, Rai A, Ahmad A, and Sastry M. (2005). Controlling the optical properties of lemongrass extract synthesized gold nanotriangles and potential application in infrared-absorbing optical coatings. Chem. Mater. 17(3): 566-572.
. Shankar SS, Ahmad A, and Sastry M. (2003). Geranium leaf assisted biosynthesis of silver nanoparticles. Biotechnol. Prog. 19(6): 1627-1631.
. Collera-Zúñiga O, Jiménez FG, and Gordillo RM. (2005). Comparative study of carotenoid composition in three mexican varieties of Capsicum annuum L. Food Chem. 90(1-2); 109-114.
. Karan T, Gonulalan Z, Erenler R, Kolemen U, and Eminagaoglu O. (2024). Green synthesis of silver nanoparticles using Sambucus ebulus leaves extract: characterization, quantitative analysis of bioactive molecules, antioxidant and antibacterial activities. J. Mol. Struct.1296: 136836.
. Kaur N, Kumar R, Alhan S, Sharma H, Singh N, Yogi R, Chhokar V, Beniwal V, Ghosh MK, Chandraker SK, and Rustagi S. (2024). Lycium shawii mediated green synthesis of silver nanoparticles, characterization and assessments of their phytochemical, antioxidant, antimicrobial properties. Inorg. Chem. Commun. 159: 111735.
. Devi N, Rani K, Kharb P, and Kaushik P. (2022). Bio-Fabrication of Euryale Ferox (Makhana) Leaf Silver Nanoparticles and Their Antibacterial, Antioxidant and Cytotoxic Potential. Plant J. 11(20): 2766.
. Chen MT, Zhang WK, Liang WL, Li YS, Li XJ, Zhu LH, and Tang HB. (2019). Controllable and extra-fast synthesis of bio-applicable silver nanoparticles with Lycium Barbarum L. aqueous extract and visible light. Mater Technol. 34(10): 581-591.
. Kotakadi VS, Gaddam SA, Venkata SK, Sarma PVGK, and Sai Gopal DVR. (2016). Biofabrication and spectral characterization of silver nanoparticles and their cytotoxic studies on human CD34+ve stem cells. Biotech. 6: 1-11.
. Akintelu SA, Bo Y, and Folorunso AS. (2020). A review on synthesis, optimization, mechanism, characterization, and antibacterial application of silver nanoparticles synthesized from plants. J. Chem. 2020: 1-12.
. Hashemi Z, Mizwari ZM, Mohammadi-Aghdam S, Mortazavi-Derazkola S, and Ebrahimzadeh MA. (2022). Sustainable green synthesis of silver nanoparticles using Sambucus ebulus phenolic extract (AgNPs@See): Optimization and assessment of photocatalytic degradation of methyl orange and their in vitro antibacterial and anticancer activity. Arab. J. Chem. 15(1): 103525.
. Khan S, Ullah I, Khan H, Rahman FU, Rahman MU, Saleem MA, Nazir S, Ali A, and Ullah A. (2024). Green synthesis of AgNPs from leaves extract of Saliva Sclarea, their characterization, antibacterial activity, and catalytic reduction ability. Z Phys Chem.