Assessing the Efficacy of Simple and Molecular Diagnostic Methods in Tracking Malaria Parasite Proliferation (P. vivax and P. falciparum)

Muhammad Zahoor; Fazli Subhani; muradali Khan; Majid Ayaz; Gul Laiba; Muhammad Junaid Ansari; Adnan Badshah; Shahzada Khurram Adrian Shah; Nauman Ahmad

Assessing the Efficacy of Simple and Molecular Diagnostic Methods in Tracking Malaria Parasite Proliferation (P. vivax and P. falciparum)

Authors

Muhammad Zahoor Department of Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, 136 Zhongshan, 2nd Rd, Chongqing 400014, China.
Fazli Subhani Department of KP, Elementary and Secondary Education Peshawar, Pakistan.
muradali Khan Department of Parasitology, College of Veterinary Sciences, The University of Agriculture Peshawar, Pakistan.
Majid Ayaz Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung, Taiwan.
Gul Laiba Department of Zoology Wildlife and Fisheries, MNS-University of Agriculture Multan, Pakistan.
Muhammad Junaid Ansari Department of Pathobiology, Faculty of Veterinary Science, BZ University, Multan, Pakistan.
Adnan Badshah Department of Parasitology, The University of Veterinary and Animal Sciences Swat (UVAS), Pakistan.
Shahzada Khurram Adrian Shah Department of Animal Health, The University of Agriculture Peshawar, Pakistan
Nauman Ahmad Faculty of Veterinary and Animal Science, Gomal University, Dera Ismail Khan, Pakistan.

Keywords:

Diagnosis, Malaria, Microscopic test, SD-rapid test, Rt- PCR analysis.

Abstract

The current research to investigate the proliferation and comparison of rapid diagnostic tests, simple microscopic tests, and molecular diagnostic techniques for P. vivax and P. falciparum parasites. A total of 1,500 residents with suspected malaria patients through medical symptom and healthy people with no malaria symptoms were included in the study. The regular age of patients was 5-35 years, range from 5 to 35 years. The majority were 90 (60%) over 25 years. The study included 17 (78.0%) males and 30 (22.0%) females. The microscopy test results identified 331 cases for malaria infection, rapid diagnostic tests (Optimal method test) results identified 345 cases for malaria infection and Plasmodium infection has been confirmed in 299 cases. The 261 positive cases according to microscopic examination samples, but 38 positive cases were negative on the microscopic and SD-rapid test examinations were recoded respectively. It is concluded that the SD-rapid test examinations showed the best correlation with microscopy diagnosing of P. falciparum and P. vivax parasites. And the high compassion and specificity of Rt-PCR examination grasp great assure in malaria parasites identification and genus separation and should be implemented as a successful surveillance instrument for malaria monitoring, control and eradication in Bannu city.

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